3d insighttm human cardiac microtissues  (Insphero Inc)

Journal: Biomimetics

Article Title: Biomimetic Cardiac Tissue Models for In Vitro Arrhythmia Studies

doi: 10.3390/biomimetics8060487

Figure Lengend Snippet: Classification of the possibility of creating cardiac tissue biomimetics depending on modeling tasks, namely, depending on the chosen pathology, using examples.

Article Snippet: , 3D , iPS cells: human cardiac microtissues (3D InSightTM Human Cardiac Microtissues from InSphero) and human biopsies , Non-adhesive plates , Non-structured microtissues; coculturing; and modeling fibrosis , Nguyen, Nhan et al. “Translational proteomics analysis of anthracycline-induced cardiotoxicity from cardiac microtissues to human heart biopsies.” Frontiers in Genetics 12 (2021): 695625. [ ] .

Techniques: Adhesive, In Vitro, Mutagenesis, Derivative Assay, Cell Differentiation

Journal: Biomimetics

Article Title: Biomimetic Cardiac Tissue Models for In Vitro Arrhythmia Studies

doi: 10.3390/biomimetics8060487

Figure Lengend Snippet: Classification of biomimetics of cardiac tissue and options for their design by examples.

Article Snippet: , 3D , iPS cells: human cardiac microtissues (3D InSightTM Human Cardiac Microtissues from InSphero) and human biopsies , Non-adhesive plates , Non-structured microtissues; coculturing; and modeling fibrosis , Nguyen, Nhan et al. “Translational proteomics analysis of anthracycline-induced cardiotoxicity from cardiac microtissues to human heart biopsies.” Frontiers in Genetics 12 (2021): 695625. [ ] .

Techniques: In Vitro, In Vivo, Injection, Derivative Assay, Polymer, Functional Assay, Agarose Gel Electrophoresis, Enzyme-linked Immunosorbent Assay, Cell Culture, Activation Assay

Journal: Communications Biology

Article Title: Network integration and modelling of dynamic drug responses at multi-omics levels

doi: 10.1038/s42003-020-01302-8

Figure Lengend Snippet: a Essential microtissue characteristics remain stable across a time period of 28 days. Contractions per 15 s, microtissue sizes and ATP content. Bars indicate measurements per week. Red line is the average over all 4 weeks. b Inverse correlation of gene body methylation and expression. Genes were classified into six classes of expression strengths based on the median fragments per kilobase of transcript per million reads (FPKM) over all time points and treatments. Methylation values ( Y -axes) were averaged for each class of genes according to the following procedure: promoters and gene bodies were binned in 75 windows, 20 bins for the promoter (−5 kb), 1 bin for the transcription start site (TSS), 4 bins for the starting exons/introns (+1 kb) and 50 bins for the rest of the gene body (TTS: transcription termination site); for each gene % methylation in each bin was averaged over all seven time points for the specific treatment and then % methylation was averaged over all genes of the respective expression strength classes. In the plots of the AC-treated experiments the respective curves for the control experiments (DMSO) are shown as dotted lines. X -axes show the prototypical gene structure. TSS: transcription start site, TTS: transcription termination site. c Enrichment of TFBSs of cardiac transcription factors in DMRs that fall into gene promoters. Odds ratios (red = higher than expected) represent the ratio of the observed number of TFBSs that fall into DMRs versus the expected number. d Over-representation of transcription factor target sets in the list of 641 dynamic response proteins (next section). X -axis represents the strength of enrichment, i.e. –log10 of the over-representation Q -value.

Article Snippet: The microtissues were cultured in 50 μl per well 3D Insight™ Human Cardiac Microtissue Maintenance Medium (InSphero, Cat #CS-07-010-01).

Techniques: Methylation, Expressing